Can Get Signal® immunostain IHC組織染色專用

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Can Get Signal 三大優點

Can Get Signal 增強免疫分析迅號的數十倍，保持較低的背景迅號
Can Get Signal 可以用在已標記過氧化物酶HRP或鹼性磷酸酶AP之相關抗體等
Can Get Signal 可以直接使用，無需稀釋

Can Get Signal® immunostain Immunoreaction Enhancer Solution

Code No. NKB-401 5 ml x 2 NKB-501 20 ml x 1 NKB-601 20 ml x 1

Description

Can Get Signal^® immunostain is a reaction solution that contains an accelerator for antigen-antibody reactions, which improves sensitivity, specificity, and S/N of immunohistochemistry (IHC) and immunocytochemistry.

Features

Improves sensitivity, specificity, and S/N of IHC. Can be applied to various detection systems (e.g., chromogenic, chemiluminescence, or fluorescence). Can be used with ABC or polymer complex methods. Solutions A and B exhibit various properties for improving results. Can be used directly without dilution (Ready-to-use).

Applications

• Immunohistochemistry (IHC)
• Immunocytochemistry

Storage condition

Store at 4ºC

Components

This kit includes the following components. All reagents should be stored at 4ºC and protected from light.

Reagent Name	Code No.
	NKB-401	NKB-501	NKB-601
Solution A	5 ml	20 ml	-
Solution B	5 ml	-	20 ml

Typical reaction flow

Flow chart of immunostaining with Can Get Signal^® immunostain

Application data

1. Detection of PCNA using paraffin-embedded sections
The localization of PCNA (proliferating cell nuclear antigen) expression in human skin was detected using paraffin-embedded sections of the human skin tissue model TESTSKINTM (Toyobo). Detection was performed by the ABC method with anti-PCNA mouse monoclonal antibody as the primary antibody and biotinylated mouse IgG as the secondary antibody. Each antibody was diluted with Solution A of Can Get Signal® immunostain prior to use. As a control experiment, PBS(-) containing 1.5% normal horse serum (conventional method) was used instead of Solution A of Can Get Signal® immunostain. As a result, Can Get Signal® immunostain produced higher signals and lower background than the conventional method.
Fig. 1. IHC detection of PCNA using paraffin-embedded tissue sections
2. Detection of paxillin with the fluorescent antibody method
The localization of paxillin in Swiss 3T3 cells was detected using anti-paxillin polyclonal antibody and Alexa488-conjugated rabbit IgG antibody. As a result, the exposure time for detection could be reduced from 3 s to 1 s by using Can Get Signal immunostain®, and the detailed fiber structure (fibrillar adhesion) could also be detected by using Can Get Signal® immunostain. However, the structure could not be detected with the conventional method due to high background signals. Fig. 2C shows a merged picture of the immunological detection with anti-paxillin and anti-phospho-tyrosine antibodies, and actin staining.
Fig.2.Immunocytochemistry detection of paxillin with the fluorescent antibody method *The data was kindly provided by Dr. Harada, Tokyo Institute of Technology. 萊恩生物科技有限公司 http://biolion.blogspot.tw 台中專員 陳俊豪 0937-229803 04-25239639